Regulatory & Policy

Henlius advances STEAP1/CD3/ CD28 trispecific T-cell engager to Phase I in prostate cancer

Shanghai Henlius Biotech (HKEX: 2696) has received clearance to initiate a Phase I clinical trial of HLX3902, a trispecific T-cell engager antibody targeting STEAP1, CD3, and CD28, in patients with metastatic castration-resistant prostate cancer and other advanced solid tumors in Australia. The trial received approval from the relevant Human Research Ethics Committee and acknowledgement from the Therapeutic Goods Administration, clearing the path for first-in-human testing of a molecule the company said has demonstrated anti-tumor activity and an acceptable safety profile in preclinical studies.

HLX3902 is designed to bind simultaneously to three distinct proteins: STEAP1, expressed on the surface of prostate cancer cells, and the T-cell surface receptors CD3 and CD28. By engaging CD3, the antibody initiates cytotoxic T-cell activation against STEAP1-expressing tumor cells — a mechanism referred to as Signal 1. The simultaneous engagement of CD28 provides co-stimulatory signaling, or Signal 2, which the company said is intended to enhance T-cell proliferation, survival, and overall anti-tumor response.

This trispecific architecture distinguishes HLX3902 from conventional bispecific T-cell engagers, which typically engage only CD3 alongside a tumor-associated antigen. By incorporating CD28 co-stimulation into the same molecule, Henlius is attempting to address a recognized limitation of CD3-only engagers: insufficient T-cell persistence and activity in immunosuppressive solid tumor environments.

STEAP1 — Six-Transmembrane Epithelial Antigen of the Prostate 1 — is a cell-surface protein overexpressed in prostate cancer and, to varying degrees, in other solid tumors including colorectal, bladder, and lung cancers. Its restricted expression in normal tissues makes it an attractive target for tumor-directed immunotherapy. Henlius noted that as of the announcement date, no trispecific antibody targeting STEAP1, CD3, and CD28 has been approved globally, positioning HLX3902 as a novel entry into this mechanistic class.

Competitive context

Approved options for prostate cancer now include second-generation androgen receptor pathway inhibitors such as enzalutamide and abiraterone, PARP inhibitors including olaparib and niraparib in combination with abiraterone for patients with homologous recombination repair alterations, the PSMA-targeted radioligand therapy lutetium-177 vipivotide tetraxetan, and the alpha-emitting radium-223 dichloride for bone-predominant disease. Despite this range of agents, patients who progress through multiple lines of therapy have limited options, and resistance mechanisms remain incompletely understood.

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T-cell engager approaches have attracted interest as a mechanistically distinct strategy in this setting. Amgen's xaluritamig, a STEAP1-by-CD3 bispecific antibody currently in Phase II development, has generated early clinical data in mCRPC and represents the most direct competitive reference point for HLX3902. Amgen also has pasritamig, a PSMA-by-CD3 bispecific, in Phase Ib/II combination studies. Regeneron is evaluating a PSMA-by-CD28 co-stimulatory bispecific, REGN4336, in combination with cemiplimab in early-phase trials.

Henlius stated that preclinical studies of HLX3902 demonstrated anti-tumor efficacy and a safety profile the company described as acceptable without disclosing specific data. The trial has been registered as NCT07533708 on ClinicalTrials.gov, listed as a Phase Ia investigation in mCRPC and other advanced solid tumors, with a projected start date of May 2026 and an estimated primary completion date of June 2027.


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