Discovery

University of Alabama at Birmingham backed with USD 2.34m NIAID grant for congenital syphilis trial

The University of Alabama at Birmingham has received a USD 2.34 million NIAID U01 cooperative agreement to conduct CONSISTENT, a Phase IV randomized controlled trial testing oral amoxicillin against benzathine penicillin G (BPG) for possible congenital syphilis in neonates — the first prospective head-to-head comparison of these regimens in this population.

Addressing a supply-chain vulnerability in standard of care

Congenital syphilis rates have risen across every US region over the past decade, and the current standard treatment — a single intramuscular dose of BPG — faces intermittent supply shortages that create clinical management gaps. CONSISTENT is designed to determine whether a 10-day course of oral amoxicillin (50 mg/kg twice daily) produces serologic responses non-inferior to BPG within six months of treatment, using a 10% non-inferiority margin.

The trial will enroll 374 neonates of at least 35 weeks gestational age across 12 US clinical sites over two years. Principal investigators are Jodie Dionne-Odom and David W. Kimberlin at the University of Alabama at Birmingham, based within the Children's of Alabama healthcare system.

Trial design and secondary aims

Beyond the primary efficacy comparison, the study incorporates an optional pharmacokinetic substudy in 30 participants to confirm that the 50 mg/kg amoxicillin dose maintains plasma concentrations above the established minimum inhibitory concentration of 0.42 mg/L for Treponema pallidum. A third aim evaluates quantitative PCR testing of oropharyngeal and nasal swabs as a diagnostic tool for refining the classification of possible congenital syphilis in exposed infants — a population currently defined by in-utero syphilis exposure with negative clinical and serologic evaluation.

The open-label design reflects the practical difficulty of blinding intramuscular versus oral administration in neonates, and the Phase IV designation indicates the study is evaluating an established antibiotic in a new indication and dosing context rather than a novel compound.

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Funding context

The first-year award of USD 2.34 million — comprising USD 1.64 million in direct costs and USD 694,000 in indirect costs — was issued by the National Institute of Allergy and Infectious Diseases on May 12, 2026, under funding opportunity PAR-24-100. The project is structured to run through April 2031. NIAID's use of the U01 cooperative agreement mechanism signals active agency involvement in trial oversight, consistent with the multicenter infrastructure the study requires.

The award reflects sustained NIH attention to congenital syphilis as a domestic public health priority at a time when case rates have continued to climb despite existing prevention and treatment guidelines.


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