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Shape and Rett Syndrome Research Trust advance AI-designed RNA editor for R168X mutation

Shape and Rett Syndrome Research Trust advance AI-designed RNA editor for R168X mutation

Seattle-based Shape Therapeutics and the Rett Syndrome Research Trust (RSRT) announced a partnership to advance SHP-401, an investigational RNA editing gene therapy targeting the MECP2 R168X mutation, the single most common point mutation in Rett syndrome, accounting for approximately 10% of cases. Under the agreement, RSRT will fund translational studies in non-human primates (NHP) to evaluate biodistribution and on-target editing, with the goal of generating data to support IND-enabling studies. Financial terms were not disclosed.

SHP-401 uses Shape's RNAfix guide RNA platform, in which an AI-designed guide RNA recruits native adenosine deaminase acting on RNA (ADAR) enzymes to correct the R168X mutation at the RNA level, restoring full-length MeCP2 protein without permanent DNA alteration. The therapy is delivered intravenously via Shape's proprietary blood-brain barrier (BBB)-penetrant AAV5-derived capsid. In a preclinical mouse study posted to bioRxiv, Shape reported approximately 70% RNA editing of the R168X mutation throughout the brain following a single IV dose, with median lifespan extended from 50 days in untreated animals to as long as 174 days and a reported 88–93% reduction in risk of death. Transcriptome-wide profiling found no evidence of meaningful off-target editing, the company said. The NHP studies funded under this agreement will evaluate the AAV5-derived capsid's CNS biodistribution and on-target editing performance as the primary translational question before IND-enabling work begins.

The RSRT has pursued a portfolio approach to Rett syndrome genetic medicines, funding multiple competing platforms simultaneously. The trust previously provided a total of USD 9.1 million to Netherlands-based ProQR Therapeutics (Nasdaq: PRQR) to advance AX-2402, an RNA editing oligonucleotide targeting MECP2 mutations via its Axiomer platform — a chemically delivered, non-viral approach that contrasts with Shape's AAV-based delivery. RSRT also partnered with Apertura Gene Therapy in April 2025 to evaluate a transferrin receptor 1-targeted AAV capsid for Rett syndrome delivery, and with Emeryville, California-based Profluent Bio in November 2025 to design AI-engineered base editors targeting recurrent MECP2 mutations. The Shape collaboration adds an AAV-delivered ADAR RNA editing approach to that portfolio, distinct from both the gene replacement strategies in late-stage clinical development and the base editing programs at earlier stages.

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The competitive backdrop for SHP-401 includes two AAV-based MECP2 gene replacement therapies in pivotal or registrational trials: Neurogene Inc.'s (Nasdaq: NGNE) NGN-401, which completed dosing in its Embolden registrational trial with topline data expected in the second half of 2027, and Taysha Gene Therapies' (Nasdaq: TSHA) TSHA-102, currently enrolling in the REVEAL pivotal trial. Both deliver functional MECP2 transgenes rather than correcting the endogenous mutation, and both require transgene regulation technology to manage dosage sensitivity. Shape's RNA editing approach corrects only the R168X mutation at the transcript level and is restricted to patients carrying that specific variant, limiting addressable population but avoiding the overexpression toxicity risk that has shaped the design of competing programs. Shape's AAV capsid technology has separately attracted commercial interest: in September 2025, Netherlands-based VectorY Therapeutics licensed Shape's BBB-penetrant AAV5-derived capsid for neurodegenerative disease programs in a deal worth up to USD 1.2 billion in milestones.


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